Journal: Oncology Letters

Article Title: Dpy-19 like 3-mediated C -mannosylation and expression levels of RPE-spondin in human tumor cell lines

doi: 10.3892/ol.2017.6465

Figure Lengend Snippet: RPESP is expressed in certain colon cancer cell lines. (A) Expression of RPESP mRNA in human tumor cell lines. Total RNAs were isolated from A549 lung adenocarcinoma, ES2 ovarian cancer, HepG2 hepatoma, HT1080 fibrosarcoma, HT29 colon cancer, Jurkat leukemia, LNCaP prostate cancer and WM266-4 melanoma cells and RT-sqPCR analysis was performed. (B) Expression of RPESP mRNA in human colon cancer cell lines. Total RNAs were isolated from human colon cancer cell lines, HT29, COLO 205, CW-2, HCT116 and LoVo, and RT-sqPCR analysis was performed. RT-sqPCR, reverse transcription-semi-quantitative polymerase chain reaction; RPESP, RPE-spondin.

Article Snippet: Human ES2 (which was kindly donated by the Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan) ovarian cancer and HCT116 (RIKEN BioResource Center) colon cancer cell lines were cultured in DMEM supplemented with 10% (v/v) heat-inactivated FBS, 100 U/ml penicillin G, 100 mg/l kanamycin, 600 mg/l L-glutamine, and 2.25 g/l NaHCO 3 at 37°C in a humidified incubator with 5% CO 2 .

Techniques: Expressing, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncogene

Article Title: TWIST1 induces expression of Discoidin Domain Receptor2 (DDR2) to Promote Ovarian Cancer Metastasis

doi: 10.1038/s41388-017-0043-9

Figure Lengend Snippet: A, B . Matrigel invasion assays. A. ES2 and B. A2780 cells were depleted of DDR2 or control (SCRM) and added to Matrigel coated Boyden chambers (8um pore size).Representative images and quantification (Means and S.D. for 3 individual chambers per group plotted as number of cells invading through to lower surface 4 per high powered field (hpf)). Repeated with statistical significance in 3 independent experiments. Unpaired t-test, **p<0.01, ***p<0.001. C. Collagen I invasion assays of ES2 shDDR2 or control (shSCRM) cells. Representative photographs taken at day 3 and day 6. D. Quantification of collagen invasion. 20 cells per well, 3 wells per condition quantified. Mean and s.d. are shown, unpaired t-test **p<0.01

Article Snippet: ES2 shSCRM or shDDR2 cells were injected intraperitoneally (i.p.) with 10×10 6 cells in 0.5 ml of PBS into female 6- to 8- week old ( n =8 per group) NU/NU nude (Charles River) mice.

Techniques: Control, Pore Size

Journal: Oncogene

Article Title: TWIST1 induces expression of Discoidin Domain Receptor2 (DDR2) to Promote Ovarian Cancer Metastasis

doi: 10.1038/s41388-017-0043-9

Figure Lengend Snippet: A. ES2 or A2780 shRNAi-depleted of DDR2 or transduced with scrambled control (SCRM) were cultured for 6 hours under serum free conditions on 2mg/mL of collagen I, and cell free media was incubated with recombinant human fibronectin (FN). Extent of cleavage was measured at time intervals of 0.25, 1, and 16 hours by SDS Page followed by coomassie staining. B, C. Quantification of cleavage of intact FN by B. A2780 or C. ES2 plotted as a fraction of remaining intact FN as compared to control. Amount of remaining intact FN (~220 kDa) quantified by densitometry. Data from 3 independent experiments are plotted. D. Quantification of cell area when plated on fibronectin coated hydrogels for 3 hrs. Dots represent individual cell areas, with box plot overlay depicting the median and interquartile ranges of area spread. n>200 cells quantified for each group. E. Representative images of ES2 shSCRM or shDDR2 cells plated on fibronectin coated hydrogels.

Article Snippet: ES2 shSCRM or shDDR2 cells were injected intraperitoneally (i.p.) with 10×10 6 cells in 0.5 ml of PBS into female 6- to 8- week old ( n =8 per group) NU/NU nude (Charles River) mice.

Techniques: Transduction, Control, Cell Culture, Incubation, Recombinant, SDS Page, Staining

Journal: Oncogene

Article Title: TWIST1 induces expression of Discoidin Domain Receptor2 (DDR2) to Promote Ovarian Cancer Metastasis

doi: 10.1038/s41388-017-0043-9

Figure Lengend Snippet: A. A2780 cells stably expressing shRNA targeting sequence for scramble control (shSCRM) or DDR2 (shDDR2). Beta-Actin was used as a protein loading control. B–D. Representative images and quantification of Balb/c Nu mice injected IP with 7.5×106 A2780 shSCRM or shDDR2 cells. Tumor burden was assessed at 14 days post injection in the B entire peritoneal cavity C. Mesentary only D. Omentum only. N=10 mice per group. Means and s.d. Unpaired t-test, *P<0.05, **P≤0.01

Article Snippet: ES2 shSCRM or shDDR2 cells were injected intraperitoneally (i.p.) with 10×10 6 cells in 0.5 ml of PBS into female 6- to 8- week old ( n =8 per group) NU/NU nude (Charles River) mice.

Techniques: Stable Transfection, Expressing, shRNA, Sequencing, Control, Injection

Journal: Stem Cell Research & Therapy

Article Title: Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model

doi: 10.1186/s13287-024-03698-0

Figure Lengend Snippet: Tumorigenicity test of orthotopic transplantation of MSCs. A Flowchart overview of the establishment of an orthotopic xenograft tumour model in mice. B Flowchart overview of the evaluation of the tumorigenic potential of MSC transplantation. C Photographs and pathological section images of xenograft tumours established in situ. D Representative image of mice in the ES-2 injection group. E Photographs of ovaries in the AD-MSC injection group. F Photographs of ovaries in the UC-MSC injection group. G Representative pathological images of ovaries and other organs in the MSC transplantation group

Article Snippet: The ovarian cancer cell line ES-2 (iCell-h060, iCell) was used as a positive control for tumorigenicity detection.

Techniques: Transplantation Assay, In Situ, Injection